. 2010 Aug 10;8(8):e1000450. doi: 10.1371/journal.pbio.1000450

Table 1. Pharyngeal fluorescent puncta in daf-2(−) mutants contain mobile KIN-19.

	Total KIN-19::tagRFP Puncta Evaluated	Number of Animals	Boxed Area Bleached⁽¹⁾	Boxed Area Bleached⁽¹⁾	Whole Puncta Bleached⁽²⁾
			Immobile (No recovery)	Mobile (Recovery)	Mobile (No recovery or partial recovery)
wild-type	36	16	25	5	6
*daf-2(e1370)*	34	18	6	6	22

The assay was done in a blind fashion in which the identity of the samples was concealed. FRAP was performed on KIN-19::tagRFP puncta in a wild-type background between Day 12–15 and in a daf-2(e1370) background between Day 13–15 of adulthood. Recovery after photobleaching was estimated between 2.5 to 4 min after bleaching.

⁽¹⁾

This category comprises puncta where the laser beam bleached the targeted boxed area without affecting the non-targeted area to the same extent. Puncta in (1) where we observed no recovery after photobleaching were determined to contain immobile KIN-19::tagRFP protein. Conversely, KIN-19::tagRFP protein was estimated to be mobile when we observed recovery of the area bleached.

⁽²⁾

This category comprises puncta where the laser beam uniformly bleached both targeted and non-targeted parts of the puncta. We observed either no-recovery or a partial recovery in fluorescence of the whole puncta. These puncta were estimated to contain mobile KIN-19::tagRFP protein.

Mobile compared to immobile KIN-19::tagRFP puncta in daf-2(+) and daf-2(−) background: Fisher's exact test p<0.0001.