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Expression of GFP-swiggle increases cell surface localisation of MT1-MMP. Cell surface proteins of MCF7 cells transfected with MT1-MMP, and GFP (lane 1), GFP-TrxA (lane 2), GFP-s14 (lane 3), or GFP-swiggle (lane 4) were biotinylated and immunoprecipitated using an anti-biotin antibody. The presence of MT1-MMP in the immunoprecipitate (top panel) and in the input lysates (bottom panel) was detected using an anti-MT1-MMP antibody.
