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. 2010 Jul 23;11:58. doi: 10.1186/1471-2121-11-58

Figure 6.

Figure 6

GFP-swiggle inhibits internalization of MT1-MMP. MCF7 cells, transiently co-transfected with MT1-MMP and either GFP-s14 (A-H) or GFP-swiggle (I-P) were incubated with an anti-MT1-MMP antibody for 2 hours at 4°C. Cells were then washed and fixed immediately with paraformaldehyde (A, E, I and M) or warmed to 37°C for 10 (B, F, J and N), 30 (C, G, K and O) or 50 (D, H, L and P) minutes before fixation. MT1-MMP is shown in panels A to D and I to L. GFP-s14 is shown in panels E to H and GFP-swiggle in panels M to P. Bar, 5 μm.