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. 2010 Jul 28;11(1):103. doi: 10.1186/1465-9921-11-103

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Copyright ©2010 Loza et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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CD3-stimulated accumulation of T cell subsets from control and asthmatic subjects. PBL from control (filled symbols) and atopic asthmatic (open symbols) subjects were cultured 5-d with CD3+CD28 monoclonal antibody + IL-2 + anti-IL-12, and then stimulated and analyzed for cytokine production by immunofluorescence-flow cytometry. Each symbol indicates the proportion (%) and number (n) of: (A) total; (B) IL-13⁺; and (C) IFN-γ⁺T cells, expressed a percent of the respective day 0 values for each subject tested. Bars = geometric mean for each parameter. p-values from two-tailed student t-tests for differences in geometric mean between control and asthmatic subjects are shown.