Figure 3.
Similar Bcd-Hb input/output relationship on D and V sides. (A) Scatter plots of normalized Hb intensity values against raw Bcd intensity values on the dorsal (blue) and ventral (red) sides of wt embryos. The effective dissociation constant Kd for Bcd activating Hb expression on the two sides can be extracted from the plots, as illustrated by dashed lines. (B) Average input/output relationship on the dorsal (blue, with error bars) and ventral (red) sides of wt embryos. B and Kd values are background-subtracted. Green profile represents a Hill curve with cooperativity n = 5. (C) Intensity noise of Hb expression in response to the Bcd gradient. B and Kd values are background-subtracted. (D) Intensity noise of Bcd at and around cHb on the dorsal (blue) and ventral (red) sides of wt embryos. In this figure (and Fig. S1 H and S2H), noise from measurement and background is not subtracted from the Bcd intensity noise profiles.