Table 1.
Continuous pulmonary damage and inflammatory response in influenza infected Rag2-/- mice
| Group | Percentage of affected lung parenchyma | Severity of necrosis in affected bronchi | Necrosis of alveolar walls | Hyperplasia of type 2 AEC | Lymphocytic infiltrates around bronchi and vessels | Infiltrating neutrophils in airways and interstitium | Histiocytic infiltrates in alveolar walls and lumina |
|---|---|---|---|---|---|---|---|
| B6 PBS | <5% | 0 | 0 | 0 | 0.2 | 0 | 0 |
| B6 day2 | 6% | 1.8 | 1.8 | 0 | 1.4 | 1.6 | 1.6 |
| B6 day4 | 12% | 4 | 3 | 0.2 | 2.8 | 1.8 | 2.4 |
| B6 day6 | 50% | 3.6 | 3.2 | 1 | 4.6 | 1.2 | 3.6 |
| B6 day8 | 34% | 2.6 | 2 | 1.8 | 4 | 0.4 | 3.2 |
| B6 day10 | 24% | 2 | 1 | 3.2 | 4.4 | 0.2 | 3.2 |
| Rag2 PBS | <5% | 0 | 0 | 0 | 0.2 | 0 | 0 |
| Rag2 day2 | <5% | 1 | 1.6 | 0 | 1.4 | 1.2 | 0.6 |
| Rag2 day4 | 18% | 3.2 | 2.2 | 0.4 | 1.2 | 2.4 | 3.2 |
| Rag2 day6 | 60% | 4.2 | 2.8 | 1 | 0.6 | 3.6 | 4 |
| Rag2 day8 | 18% | 3.6 | 2.8 | 1.4 | 0.4 | 3.2 | 2.2 |
| Rag2 day10 | 44% | 3.4 | 3.2 | 3 | 0 | 4.4 | 3.6 |
Semi-quantitative scoring of pulmonary lesions and immune cell infiltrates (median value of each group, 3 or 4 mice per group, 5 successive sections per sample).
Lung tissue sections from female wild type or Rag2-/- mice infected with 2 × 103 FFU PR8 were prepared on the days p.i. indicated, stained with hematoxylin and eosin and evaluated for the indicated features by a semi-quantitative grading system with the scores of 0 to 5 representing the degree of severity as follows: 0 = no lesion; 1 = minimal; 2 = mild; 3 = moderate; 4 = severe; 5 = marked. Abbreviations: AEC, alveolar epithelial cell; B6, C57BL/6J mice; PBS, mice treated with Phosphate Buffered Saline as non-infected control; Rag2, Rag2-/- mice.