TABLE 2.
PSS1 and PSS2 Expression in the CNS Versus PNS1
| Tissue | PSS1 | PSS2 |
|---|---|---|
| Forebrain | 551 ± 120 | 49 ± 18 |
| Cerebellum | 341 ± 135 | 80 ± 14 |
| Tectum | 952 ± 243 | 78 ± 20 |
| Brainstem | 724 ± 136 | 355*± 127 |
| SG | 2.6*± 2.2 | 446*± 241 |
| DRG | 2.5*± 1.0 | 117 ± 146 |
| CG | 0.34*± 0.1 | 1211*± 127 |
1
Tissue was isolated from E14 chicken embryos, and transcripts encoding PSS1 and PSS2 were quantified by real-time qPCR using Taqman probes. Values for each target gene were normalized to chicken ribosomal binding protein s17 (CHRPS), yielding arbitrary mRNA units (AU) as described in Materials and Methods. Values represent the AU of three independent tissue and RNA isolations ± standard deviation.
*
These values differ from values of the same gene for forebrain, tectum, and cerebellum by P < 0.001, one-way ANOVA, Tukey’s post hoc test. CNS, central nervous system; PNS, peripheral nervous system; SG, sympathetic lumbar ganglia; DRG, dorsal root ganglia; CG, ciliary ganglia.