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. 2009 Jul 29;1(6):556–569. doi: 10.1159/000231977

Fig. 1.

Fig. 1

Western blot analysis with 6 novel cytochrome b558 antibodies. Several neutrophil fractions were submitted to SDSPAGE followed by immunoblot identification: a Neutrophil cellular extracts (40 μg/well) from either X0 CGD patient (X0) or normal subject (N), b neutrophil crude membrane (Mb: 30 μg/well). The presence of gp91-phox was detected using the 6 novel mAbs (12E6, 17A2, 16G7, 16G6, 13D4 and 13C4) or control antibodies (mAb 44.1 or a polyclonal antibody, pAb, directed against the C-terminal peptide of p22-phox). The immune complexes were detected by ECL.