Fig. 2.
S. stercoralis extract stimulates eosinophil chemotaxis using PI3K and tyrosine kinases. Eosinophils (1 × 106 cells) were added with IL–5 (30 ng/ml) to the upper chambers of a transwell plate. Chemotaxis was stimulated by incubation with either eotaxin/CCL11 (80 ng/ml), SDF–1α/CXCL12 (400 μg/ml), or S. stercoralis extract (1 mg/ml). a Eosinophils were pretreated with the PI3K inhibitor, wortmannin (1 × 10−9 to 1 × 10−7 M). b Eosinophils were pretreated with various doses of the nonspecific tyrosine kinase inhibitor herbimycin A (1 × 108 to 1 × 106 M). Data are presented as percentage of migration in relation to untreated control, represented as 100%. Bars represent the mean percentage over 4–6 experiments ± standard error of the mean. Numbers above control bars indicate the raw number of untreated migrated cells (arithmetic mean ± standard error of the mean ×103 migrated cells). * p<0.05.