Fig. 3.

Increasing doses of the p38 MAPK cascade inhibitor, SB202190, and the p42/44 MEK inhibitor, PD98059, inhibited eosinophil chemotaxis. Eosinophils (1 × 10⁶ cells) were added with IL-5 (30 ng/ml) to the upper chambers of a transwell plate. Chemotaxis was stimulated by incubation with either eotaxin/CCL11 (80 ng/ml), SDF-1α/CXCL12 (400 μg/ml), or S. stercoralis extract (1 mg/ml). Blocking p38 with SB202190 (a) and blocking p42/44 with PD98059 (b) are shown. Data are presented as percentage of migration in relation to untreated control, represented as 100%. Bars represent the mean percentage over 5–6 experiments ± standard error of the mean. Numbers above control bars indicate the raw number of untreated migrated cells (arithmetic mean ± standard error of the mean ×10³ migrated cells). * p<0.05.