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The effect of SNP or DMA on baseline cytoplasmic pH in bicarbonate-free HEPES buffer. The cells were loaded with BCECF superfused with bicarbonate-free HEPES buffer, and baseline pHi was recorded for 5 minutes. Then, 100 μM DMA or 100 μM SNP was added to the superperfusate. Control cells received an equivalent amount of DMSO. Results are shown as the mean ± SEM of five to seven individual experiments. The slow, continuous drift of baseline pHi is probably due to dye bleaching.
