Figure 2. Enrichment of the chromatin regulators in undifferentiated cell-enriched bam testis.

(A) Heat map showing genes that are uniquely expressed in both bam testis vs. wt testis, and bam ovary vs. wt ovary comparisons. Unique expression means that a certain gene is expressed in one sample (RPKM ≥1) but silent in another sample (RPKM <0.5). The RPKM cutoffs for expressed and silent genes are based on⁹⁸. Heat scale: log₂RPKM. To calculate the log₂ RPKM values of individual gene, all their original RPKM values were added by a pseudo-count of 1. (B) Enriched chromatin remodeling/modifying factors and histone modifying enzymes in bam testis. Abbreviations: PcG - Polycomb group complex, PRC1 and PRC2- Polycomb repressive complex 1 and 2, TrxG- Trithorax group complex, HMT- histone methyltransferase, HAT- histone acetyltransferase, HDAC- histone deacetylase, tTAFs- testis TAF homologs, tMAC- a testis-specific version of the MIP/DREAM complex, meiotic genes and TD- terminal differentiation genes for spermiogenesis. (C) In situ data using antisense riboprobes that recognize the trx (HMT) gene and lid (HDMT) gene in wt testis, respectively. There is a co-enrichment of the trx and lid transcripts in undifferentiated cells located at the tip of the wt testis, indicated by the black arrows. (D) In situ data using antisense riboprobes that recognize the Pcaf (HAT) gene and Rpd3 (HDAC) gene in wt testis, respectively. There is a co-enrichment of the Pcaf and Rpd3 transcripts in undifferentiated cells located at the tip of the wt testis, indicated by the black arrows.