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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Dev Biol. 2010 Jun 9;345(1):18–33. doi: 10.1016/j.ydbio.2010.05.502

Figure 3. PVD branches fasciculate with motor neuron commissures and sub-lateral nerve cords.

Figure 3

Confocal images of PVD::GFP marker (A-D,P), panneural::dsRed (E-H,P) and merged reporters (I-L,O,P) show PVD dendritic branches, motor neuron commissures (arrow head) and sub-lateral nerve cords (arrow). PVD secondary branches lie in the same plane as motor neuron commissures as shown in rotated Z-stack from PVDR [(B,F,J)(rotated 55O on the X-axis and 45O on the y-axis)]. Rotated Z-stack of left side (ventral up) shows circumferential 4O branches [(C,G,K (rotated 80O on X-axis and 90O on Y-axis)]. PVD 3O branches fasciculate with dorsal and ventral sublateral nerve cords (D,H,L,O)(anterior left, ventral down). Schematic transverse section (M) shows PVD (L+R) (black) and fasciculation of some 2O branches (left) but not others (right) with motor neuron commissures (red). Lateral view of PVDR (N,O) showing 3O branches fasciculated with sub-lateral nerve chords (arrow). PVDR fasciculates with processes in the sub-lateral nerve cords (P, arrow) but does not contact the touch neuron, PVMR (P, arrowhead). Scale bars are 10um (A-C,E-G,I-K,O) or 15 um (D,H,L,P). See supplemental movie 1.