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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Cancer Gene Ther. 2010 May 7;17(9):599–613. doi: 10.1038/cgt.2010.16

Figure 2. MTT assay and activation of signaling molecules.

Figure 2

Knockdown of MMP-9, uPAR and CB in PC3 and DU145 cells reduced cell proliferation and effect the activation on ERK and Akt. (a) Cell proliferation index of PC3 and DU145 cells transfected siRNA plasmids and controls was assessed by MTT assay. Each bar represents the mean of triplicate analysis of mean ± S.D (p < 0.05). (b) Western blot analysis showing the effect of siRNA expressing plasmids on total and phosphorylated forms of ERK and Akt in PC3 and DU145 cell lines. (c) Phsophorylation of ERK and Akt were quantified using densitometry analysis. Data represented is the mean of three different experiments and bar are mean S.D (* p < 0.01, ** p < 0.05 and # p< 0.01).