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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Cancer Gene Ther. 2010 May 7;17(9):599–613. doi: 10.1038/cgt.2010.16

Figure 6. Elucidation of apoptotic pathway.

Figure 6

siRNA mediated down regulation of MMP-9, uPAR and CB induces apoptosis by activating FAS, FADD, cleavage of caspase-8 and caspase-7. (a) After 72 h, total cell lysates collected from control and transfected cells were subjected to SDS-PAGE analysis and transferred onto nitrocellulose membrane. The membranes were immunoblotted overnight by incubating with Bcl-2, Bax, FAS-ligand, FAS, FADD, XIAP, caspase-8 and caspase-7 specific antibodies. * and ** represents two cleaved forms of capsase-8 (44/42 kDa and 18 kDa forms, respectively). Signal detection was carried out by ECL reagent and autoradiography with X-ray film. GAPDH antibodies were used to confirm equal loading of proteins in each lane. (b) Band intensity of respective proteins were quantified by densitometry analysis and normalized with GAPDH levels as shown in the bar graph (mean S.D; p<0.05).