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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Traffic. 2010 Jun 15;11(9):1141–1150. doi: 10.1111/j.1600-0854.2010.01088.x

Figure 3. pHluorin used for quantification of steady-state levels of extravacuolar cargo by fluorescence microscopy.

Figure 3

Fluorescence intensity (in arbitrary units, a.u.) of WT, ΔΔΔΔ + Ent1 and ΔΔΔΔ + ENTH1 cells expressing Ste3-GFP (A) or Ste3-pHluorin (B). Values are presented as mean ± s.e.m., with the number of measured cells indicated in parentheses (One-way ANOVA with Tukey’s Multiple Comparison post-hoc test, ***p<0.001). (C) Western immunoblot of cell extracts from WT, ΔΔΔΔ + Ent1 and ΔΔΔΔ + ENTH1 strains tagged at the endogenous STE3 locus with either GFP or pHluorin and probed with anti-Ste3 or anti-GAPDH antibodies as indicated.