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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Traffic. 2010 Jun 15;11(9):1141–1150. doi: 10.1111/j.1600-0854.2010.01088.x

Table 2.

Plasmids used in this study

Plasmid Description Details Source
pBW0659 pFA6a-GFP(S65T)-kanMX6 C-terminal GFP(S65T)-KAN integrating plasmid M.S. Longtine (16)
pBW0768 pENT1 (TRP1) pRS414::ENT1 (TRP1, CEN) Laboratory plasmid
pBW0778 pENTH1 (TRP1) pRS414::ent1 (aa1-151) (TRP1, CEN) Laboratory plasmid
pBW1571 pFA6a-pHluorin-kanMX6 C-terminal Super-ecliptic pHluorin-KAN integrating plasmid This study
pBW1679 pFA6a-pHluorin-natMX4 C-terminal Super-ecliptic pHluorin-NAT integrating plasmid This study
pMB103 pVPS4E233Q pRS416::VPS4E233Q (URA3, CEN) M. Babst
pMB243 pIST1-mCherry (URA3) pRS426::Ist1-mCherry (URA3, 2μ) M. Babst (25)
pCI-super-ecliptic synaptopHluorin Synaptobrevin-pHluorin mammalian expression plasmid G. Miesenbock (6)