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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Traffic. 2010 Jun 2;11(9):1151–1167. doi: 10.1111/j.1600-0854.2010.01085.x

Fig. 3.

Fig. 3

Pitpnb immunolocalization in the adult zebrafish retina. (A) Immunolocalization of Pitpnb. An adult retina cryosection labeled with the anti-Pitpnb serum revealed Pitpnb expression is confined to the photoreceptor layer (PL) and the outer plexiform layer (OPL). (B) A negative control section labeled with pre-immune serum shows no staining. (C) A double-label experiment. Retinal sections dual-labeled for Pitpnb (green; anti-DrPITPβ antibodies) and the zpr-3 marker (red; mAb zpr-3; revealed Pitpnb is not expressed in rods. (D) A dual-label image shows staining profiles for Pitpnb (red) and α-tubulin (green). (E) A high magnification image of a Pitpnb staining profile revealing the intense signal in the photoreceptor synaptic pedicles (arrows) in the OPL. (F) Expression of the zpr-1 staining profile (visualized via the mAb zpr-1 antibody) specifically labels double cone cells. (G) A dual-label image. The merged Pitpnb (green) and zpr-1 staining profiles (red) from frozen retinal sections are shown. Arrowheads in Panels F and G point to the double-labeled cone cell synaptic pedicles. Abbreviations: PL, photoreceptor layer; INL, inner nuclear layer; GCL, ganglion cell layer; ROS, rod outer segments; OPL, outer plexiform layer; IPL, inner plexiform layer; DCOS, double cone cell outer segments. Scale bars are 50 µm in all panels except Panel E (25 µm).