Fig. 6.

Pitpnb morphants present double cone cell structural defects. (A) Five-base-mismatch control (MO5-M) and Pitpnb morphants (3dpf) were fixed and dehydrated. After embedding in Polybed 812 resin, 3µm sections were cut and stained with 1:1 methylene blue:Azure II. No gross structural defects are observed in morphant eye sections relative to control. Scale bars -- 35 µm. (B) Ultrathin sections were stained with uranyl acetate and lead citrate, and visualized by electron microscopy. Outer cone cells are clearly recognized in the control by their well-formed and distinctive outer-segments (indicated by red arrows). These structures are absent, or otherwise unrecognizable, in the morphant retinas. Scale bars -- 2 µm.