Fig. 7.

Identification of zpr-1 antigen as arrestin-3-like. (A) Lysate of adult zebrafish eye was fractionated by SDS-PAGE, the resolved species transferred to nitrocellulose, and zpr-1 antigen identified by immunoblotting with mAb zpr-1. (B) mAb zpr-1 antigen was immunoisolated from adult zebrafish eye lysates and resolved by SDS-PAGE. Two controls were included: sample without lysate, and sample without mAb zpr-1. The species indicated with an arrow was uniquely recovered from the complete incubation. (C) Recombinant zebrafish β-actin and Arr3L cross-reactivity with mAb zpr-1 or anti-actin immunoglobulin was tested by blotting -- as indicated. Naïve bacterial lystaes served as negative control. (D) Embryos (1–4 cell stage) injected with morpholino against Arr3L (MO1), or the corresponding 5-base Arr3L mismatch control morpholino (MO5-M), were examined at 3 dpf for Arr3L, green opsin, and Pitpnb staining. Uninjected (mock) served as additional control. Scale bars -- 100 µm. (E) Arr3L morpholino-injected (MO1) and uninjected control embryos were fixed and prepared for ultra-thin section electron microscopy. Sections from at least 3 fish were examined and representative images are shown. Outer segments are indicated with a red arrow. Scale bars -- 2 µm.