Table 5.

Effect of rad52 SUMOylation-defective mutants on meiotic recombination

Genotype	Meiotic recombination frequency (×10−4)a	Fold change relative to wild-type	Sporulation efficiencyb, %	Germination efficiencyc, %	Relative viabilityd
RAD52	118 ± 31	1	41	96	1
rad52Δ	0.06 ± 0.06	0.001	1	n.a.	0.10
K43,44R	144 ± 38	1.2	58	69	0.95
K253R	77 ± 24	0.7	55	88	0.84
K43,44,253R	109 ± 27	0.9	53	96	2.39

^aRecombination frequency is presented as events per colony forming unit. For each strain, three trials were performed. Strains ML412 (wild type), ML414 (rad52Δ), ML466 (rad52-K43,44R), ML467 (rad52-K253R), ML480 (rad52-K43,44,253R) were sporulated on solid medium at 30°C essentially as described previously (39). [See schematic in Table 1(i)].

^bSporulation efficiency was determined as the fraction of three to four spore tetrads out of all cells after 3 days on solid SPO medium at 30°C.

^cGermination efficiency was determined by tetrad dissection of four-spore tetrads after 3 days on solid SPO medium at 30°C.

^dViability is the number of colony forming units per milliliter per OD₆₀₀ after sporulated on solid medium at 30°C.