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. 2010 Apr 5;38(14):4586–4598. doi: 10.1093/nar/gkq214

Figure 7.

Figure 7.

CdnL and CarDNter physically interact with M. xanthus RNAP. (A) Analysis of RNAP retention by CdnL-His6 immobilized on TALON metal affinity resin. Shown are silver-stained 10% SDS–PAGE gels of the flowthrough (FT), low salt (50 mM) and high salt (500 mM) eluates on passing M. xanthus core RNAP through a column of TALON resin alone (‘No His6-protein’), TALON bound to His6-DksA as the positive control, or TALON bound to CdnL-His6. The α, β and β′ RNAP subunits are indicated. (B) Bacterial two-hybrid analysis of the interaction of M. xanthus RNAP β19–148 region (in plasmid pUT18C) with the TRCF segment (514–645), CdnL, or CarDNter (in pKT25) as indicated. Cells expressing only one fusion protein are negative controls.