Figure 6.

Specificity of pRNA detection demonstrated by using total RNA from the B. subtilis wild-type (wt) strain PY79 (lanes 4 and 5) versus a 6S-1 RNA knockout derivative strain, PY79 ΔbsrA (lanes 6 and 7); lanes 4 and 6, total RNA from cells harvested in stationary phase; lanes 5 and 7, total RNA from cells harvested during outgrowth (for details, see ‘Materials and Methods’ section). 5S rRNA was used as an internal loading control in lanes 4–7; since we used native PAGE here, the expanded area of 5S rRNA signals may be due to different 5S rRNA conformers.