Table 1. miRNAs altered temporally after preconditioning.
| miRNA | 6h | 24h | 72h | # of targets |
|---|---|---|---|---|
| Up-regulated | ||||
| mir-374 | 9.48 | 3.51 | 2.76 | 260 |
| mir-98 | 6.98 | 2.95 | 2.91 | 280 |
| mir-340 -5p | 6.01 | 2.00 | 1.67 | 419 |
| mir-21 | 5.79 | 13.03 | 5.71 | 195 |
| let-7a | 4.01 | 1.83 | NC | 367 |
| mir-352 | 3.77 | 1.65 | 2.58 | 133 |
| mir-379* | 3.47 | 1.84 | 1.70 | -- |
| mir-335 | 3.35 | 1.79 | 1.61 | 332 |
| mir-181b | 2.63 | 1.51 | 1.62 | 396 |
| mir-26b | 2.18 | 1.53 | 1.76 | 231 |
| mir-181d | 2.06 | 2.22 | NC | 420 |
| mir-15b | 1.56 | 2.18 | 4.07 | 513 |
| mir-146a | 1.96 | 1.57 | 2.70 | 287 |
|
| ||||
| Down-regulated | ||||
| mir-466c | 20.05 | 27.18 | 20.38 | 209 |
| mir-292 -5p | 12.37 | 7.11 | 7.24 | 235 |
| mir-328 | 7.41 | 1.58 | 1.87 | 271 |
| mir-873 | 7.21 | 8.94 | 9.64 | 328 |
| mir-494 | 5.31 | 2.63 | 4.40 | 340 |
| let-7d* | 4.77 | 1.65 | 4.42 | -- |
| mir-345 -5p | 4.41 | 2.04 | 2.01 | 138 |
| mir-92b | 4.06 | 2.13 | NC | 187 |
| mir-30c-2* | 3.83 | 2.02 | 3.01 | -- |
| mir-322* | 1.84 | 2.15 | 2.52 | -- |
The values are mean fold increases or decreases in the PC groups over sham group obtained from 36 cross-comparisons (n = 6 for each group) in each case. The SD was <15% in all cases. Only the fold changes that are statistically significantly different from sham (by ANOVA) were given. NC, no change compared to sham. The # of targets for each miRNA was predicted using the web tool microRNA.org. The miRNAs are transcribed as long pri-miRNAs (primirs) with several stem loop structures. The RNAse Drosha cuts a primir to release the ~70 nt stem loop structures called pre-miRNAs (premirs). Another RNAse Dicer releases a mature miRNA (~22 nt long) from a premir. In some cases, a second miRNA can be formed from the other arm of a premir. The two miRNAs formed from the same premir are usually given the same number, but the minor one will be denoted with a star. The limitation of the et prediction algorithms like microRNA.org is that they can not predict the targets of miRNAs with stars.
Hence, the miRNAs with a were not used in the pathways analysis.