Figure 3.

RIP1 and RIP3 are expressed in preOLs and their expression level is not affected by arachidonic acid treatment. (A). RT-PCR analysis of RIP1 transcript in cultured preOLs. RT, reverse transcrip-tase. (B). Western blotting analyses of RIP1/3 protein levels. PreOLs were treated with AA (100μM) in the presence and absence of Nec-1 (20μM) for 1 or 5 h. AA treatment did not change RIP1/3 expression. Nec-1, which inhibits RIP1 kinase activity and prevents AA toxicity, also had no effect on RIP1/3 expression.