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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Anal Biochem. 2009 Sep 19;397(1):24–28. doi: 10.1016/j.ab.2009.09.031

Fig. 2.

Fig. 2

The influence of various concentration of the ion-pair reagent (HXA) on the RPIP-UPLC separation of eight different CS disaccharide standards. Experimental conditions: an ACQUITY UPLC™ BEH C18 column (Waters, 2.1 × 150 mm, 1.7 μm) using solution A for 10 min, followed by a linear gradient from 10 to 40 min of 0% to 50% solution B. Solution A and B for UPLC were 0 % and 75 % acetonitrile, respectively, containing HXA (5, 15 and 40 mM, respectively, in panel A, B and C) as an ion-pair reagent and a fixed amount of HFIP (100 mM in panel A, B and C) as a buffering acid; detection wavelength 232 nm; flow rate 100 μl/min; injection volume 5 μl.