Fig. 2.

GAK is required for correct spindle assembly. a U2OS cells were transfected with indicated siRNAs. Twenty-four hours after transfection cells were re-transfected and 48 h after the first transfection cells were fixed so that spindles and DNA could be visualized by anti-α-tubulin staining and DAPI, respectively. b Cells were treated as in (a), and the spindle length was measured using the α-tubulin staining. Graph in (b) shows averages of three independent experiments with 25 cells scored per condition per experiment. Error bars represent standard deviations. Scale bar in (a) indicates 5 µm