Headspace GC-MS analysis. (A) Chromatograms of headspace-collected volatiles of female neotenics, eggs, workers, and nymphs. Female neotenic and egg volatiles consisted of identical compounds, nBnB and 2M1B, whereas workers and nymphs did not have these compounds. STD, toluene as an internal standard. (B) Mass spectra of nBnB from female neotenics (Upper) and of commercial origin (Lower). (C) Mass spectra of 2M1B from female neotenics (Upper) and of commercial origin (Lower).