Suppression of the differentiation of new female neotenics by a blend of synthetic nBnB and 2M1B. (A) Experimental setup for the inhibitory bioassay of synthetic volatiles. (B) Inhibitory effect of an artificial pheromone blend. A 2:1 mixture of nBnB and 2M1B suppressed the differentiation of new female neotenics (P < 0.001, Tukey's HSD), whereas n-butyl acetate, an ester (control 2), did not (P = 0.82) compared with control 1 (distilled water). (C) Comparison of inhibitory effect of the individual compounds and a 2:1 mixture of nBnB and 2M1B. The blend significantly suppressed the differentiation of new female neotenics (P < 0.001, Tukey's HSD), whereas neither nBnB alone (P = 0.97) nor 2M1B alone (P = 0.99) had a significant effect compared with the control (distilled water). Values denote the mean ± SEM of five replications.