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. 2010 Jul 20;107(29):13046–13050. doi: 10.1073/pnas.1002396107

Fig. 3.

Fig. 3.

Mutant SOD1–induced caspase-1 activation is counteracted by autophagy. (A–C) Primed cells were stimulated with WT or G93A-SOD1 as indicated. (A) Immunoblot analysis of cytoplasmic and organelle fraction of BMMs stimulated with G93A-SOD1 (2 μM) for 6 h in the presence of 3-methyladenine (3MA) using antibodies against human SOD1, GAPDH (cytoplasmic marker protein), and LAMP1 (organelle marker protein). (B) ELISA of SOD1 (10 μM) induced mature IL-1β from BMMs in the presence of wortmannin or 3-methyladenine. (C) Caspase-1 activation determined by flow cytometry analysis with caspase-1 FLICA in autophagy-related 5–deficient (ATG5−/−, ○) and nondeficient ATG5+/+ (•) mouse embryonic fibroblasts (MEFs) transfected with the indicated concentrations of SOD1. Data are representative of three independent experiments; error bars represent SEM of triplicate wells.