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. 2010 Jun 21;107(29):12828–12833. doi: 10.1073/pnas.0910885107

Fig. 2.

Fig. 2.

The p53 induction is ROS and MMP-independent. (A) Western analysis of p53 levels in RKO cells exposed to 2 μM antimycin A (ANT), 2 μM myxothiazol (MXT), 1 μM stigmatellin (STG) for 18 h, or without treatment. Where indicated 10 mM NAC was added 1 h before the treatment. (B) Western analysis of p53 levels in RKO cells exposed to 300 μM H2O2 for 20 h. Where indicated 10 mM NAC was added 1 h before the treatment. (C) Western analysis of p53 levels in RKO cells exposed for 18 h to 2 μM myxothiazol (MXT) or uncoupling reagents 0.4 mM dinitrophenol (DNF), 5 μM TTFB, 1 μM FCCP, 1 μM SF or not treated (C). (D) Luciferase assay for p53-dependent transcription in HeLa cells treated for 20 h, as in A. (E) Luciferase assay for p53-dependent transcription in HeLa cells exposed for 20 h to the reagents, as in C.