Skip to main content
. 2010 Jun 29;107(29):13057–13062. doi: 10.1073/pnas.0914501107

Fig. 3.

Fig. 3.

ERα stimulates insulin promoter activity independently of the ERE. (A) Effect of E2 on insulin promoter activity in vivo. Five-week-old prepubertal female RIP-Luc transgenic mice were injected with E2 (100 μg) 24 h before islet isolation and measurement of luciferase activity from islet homogenates (n = 3–4/group). (B) Effects of glucose and E2 on insulin promoter activity in vitro. INS-1 cells were transfected with RIP-Luc. Relative luciferase activity was measured after 6 h of treatment with indicated glucose concentrations in the absence or presence of E2 (10−8 M). (C) Effects of E2 (10−8 M) and actinomycin D (2 × 10−6 M) treatment for 48 h on insulin concentration in WT islets (n = 3–5/group). (D) Effects of E2 (10−8 M) treatment for 48 h on preproinsulin expression in WT, αERKO−/−, and αERKOAA/- islets (n = 3–6/group). V, vehicle. Results represent mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001.