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. 2010 Aug 11;2(4):e00040. doi: 10.1042/AN20100015

Figure 3. Hsp70 is required for KU-32 to protect against neuregulin-induced demyelination.

Figure 3

Myelinated mouse SC-DRG neuron co-cultures were prepared from WT (a–c) or Hsp70 KO (d–f) mice and treated overnight with vehicle or 1 μM KU-32. The cultures were treated with PBS or 200 ng/ml NRG1 for 4 days and myelin segments were visualized by staining the cultures for MBP. Total cell number was assessed by staining nuclei with DAPI. Cell profiler was used to calculate the total segment area and internode length from six fields per six coverslips per treatment. Arrows show examples of internode length. The results are expressed as fold of the untreated control and are the average of three experiments per genotype. *P< 0.05 versus KU-32, ∧P<0.05 versus NRG1.