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. 2010 Jul 13;103(3):401–410. doi: 10.1038/sj.bjc.6605763

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Copyright © 2010 Cancer Research UK

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C35-expression leads to invasive phenotype, associated with epithelial to mesenchymal transition. (A) Schematic illustration of the invasion assay set-up in a collagen gel containing fibroblasts overlaid with epithelial cells. (B) H16N-2 cells expressing empty vector (null, left panels), C35-expressing pool (middle panels) and C35hi-expressing cells were stained by immunohistochemistry for C35 and E-cadherin. Note specific areas of E-cadherin loss in C35 pool (arrows). (C) Quantification of C35 and E-cadherin by AQUA is shown. Bar indicates mean of measurements (P-value indicators: ^*<0.05; ^**<0.01; ^***<0.001).