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. Author manuscript; available in PMC: 2011 Apr 2.
Published in final edited form as: Circ Res. 2010 Feb 18;106(6):1117–1128. doi: 10.1161/CIRCRESAHA.109.212530

Figure 3. AMPKα2 deletion increases NAD(P)H oxidase expression via NF-κB activation in MAECs.

Figure 3

Lysates from WT and AMPKα2−/− MAECs were subjected to western blot analysis for (A) p47phox, p67phox, and gp91phox, as well as (B) IκBα and phosphorylated IκBα. The blots are representative of blots obtained from three independent experiments. *P<0.05 vs. WT. (C) Western blot analysis of the NF-κB subunits, p50 and p65, in nuclear fraction of MAECs (n=3). *P<0.05 vs. WT. (D) The expressions of p47phox and p67phox in AMPKα2−/− MAECs treated with an NF-κB translocation inhibitor (peptide sequence: AAVALLPAVLLALLAPVQRKRQKLMP, 50 mg/ml) for 24 h (n=3). *P<0.05 vs. control. (E) p47phox and p67phox expressions in MAECs (WT and AMPKα2−/−) incubated with the proteasome inhibitor, MG132 (0.5 µM), for 4 h (n=3). *P<0.05 vs. WT control, #P<0.05 vs. AMPKα2−/− control.