(A) Representatives of immunohistochemical staining and quantifications for 3-NT, MDA, and HNE positive proteins (original magnification ×400) in aortas from LDLr−/− and LDLr−/−/AMPKα2−/− mice. *P<0.05 vs. LDLr−/− control mice, #P<0.05 vs. LDLr−/−/AMPKα2−/− control mice. (B) The ROS productions were detected by DHE/HPLC in LDLr−/− and LDLr−/−/AMPKα2−/− mice aortas. *P<0.05 vs. LDLr−/− control mice. (C) The levels of 3-NT were measured by Western blot. *P<0.05 vs. LDLr−/− control mice, #P<0.05 vs. LDLr−/−/AMPKα2−/− control mice. (D) Effects of MG132 on endothelium-dependent vasorelaxation in LDLr−/− and LDLr−/−/AMPKα2−/− mice aortas. *P<0.05 vs. LDLr−/− control mice, #P<0.05 vs. LDLr−/−/AMPKα2−/− control mice. n=5–6 per group.