Effects of PH on mRNA levels of progenitor markers were examined in all 102 mice described in Figure 1. (A) QRT-PCR analysis of Fn14, Tweak, Afp. Results are expressed as fold change relative to 0h liver tissues; mean ± SEM are graphed. Data are grouped into pre-replicative, replicative and post-replicative sets for statistical analysis. *P<0.05 or **P<0.01 or ***P<0.001 relative to time 0h (ANOVA). (B) Two other progenitor markers, AE1/AE3 and Mpk2, were evaluated using immunohistochemistry in 0h and post-PH livers from randomly selected mice that were sacrificed at either 12h, 24h or 48h post-PH (n=4 mice/time point). Representative sections are displayed. AE/AE3 or Mpk2-stained cells are brown.