Table 2.
Primers used in the PCR amplification to obtain the optimized SAG1(77-322) sequence.
| Primer name | Length | Sequences |
|---|---|---|
| AC | 26 bp | 5' atcgatatgggcaacttgagatcttc 3' |
| ERV | 39 bp | 5' gatatcTCAcatAgcaaaGatAgaaacAtgaGAAgctgt 3' |
| SK | 57 bp | 5' cccgggtcatagctcatctttctcagacatAgcaaaGatAgaaacAtgaGAAgctgt3' |
| F1 | 36 bp | 5' tactcaccAaacagAcaaatctgTccagcTggtact 3' |
| R1 | 54 bp | 5' TctgttTggtgagtaAgcaagagtTggaggTtctgtAagAgctgtCttaggAca 3' |
| F3 | 30 bp | 5' acaacTcaAacAtttgtTgtTggttgcatc 3' |
| R3 | 30 bp | 5' aaaTgtTtgAgttgtAacTggAaacttctc 3' |
| F4 | 33 bp | 5' caagcTagagcctcatcTgtTgtcaaCaatgtc 3' |
| R4 | 33 bp | 5' AgatgaggctctAgcttgAactgtcacTgtgac 3' |
| F5 | 39 bp | 5' ggaccAactacaatgacTctTgtTtgTggTaaagatgga 3' |
| R5 | 36 bp | 5' AagAgtcattgtagtTggtccttcAgcagacaactt 3' |
| F7 | 48 bp | 5' gtTatCattggatgTacaggTggatcTcctgagaagcatcactgtacT 3' |
| R7 | 39 bp | 5' AcatccaatGatAacActCttAgaTtcAgctggaaatgc 3' |
| F8 | 48 bp | 5' ggAgctgcaggAtcagcTaaGtcTgctgcAggaacagcTTCtcaTgtttcT 3' |
| R8 | 48 bp | 5' tgaTcctgcagcTccAgcaaactcAagtttcacAgtacagtgatgctt 3' |
Recognition site for the endonuclease ClaI, EcoRV and SmaI indicated underlined in AC, ERV and SK primers, respectively. Specific mutation sites are indicated in capital letters.