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. 2010 Jul 23;6:43. doi: 10.1186/1744-8069-6-43

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Copyright ©2010 Hossaini et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Arc/Arg3.1 is only expressed in the superficial dorsal horn. Light micrographs showing the distribution of neurons expressing Arc/Arg3.1 mRNA (A, B) or protein (C) in the rat spinal dorsal horn after peripheral stimulation with 25% mustard oil gauze wrapped around one hind paw for 2 hours. Note that many of the Arc/Arg3.1 labeled neurons are located in laminae I&II, and very few labeled neurons are present below lamina II. D, Fluorescent micrograph showing the expression of Arc/Arg3.1 mRNA and its colocalization with the neuronal marker NeuN. Arrowheads indicate Arc/Arg3.1 mRNA and NeuN double labeled neurons. Scale bar: 50 μm (A and D); 25 μm (B); 100 μm (C).