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. Author manuscript; available in PMC: 2010 Aug 11.
Published in final edited form as: Nat Protoc. 2008;3(4):674–678. doi: 10.1038/nprot.2008.35

Figure 2.

Figure 2

Images of different stages of the patterning process. (a) PDMS stamp (inset: vertical section through one post); (b) molded collagen gel; (c) molded gel during addition of cells (note that cells are both in the wells and on top of the gel); (d) molded gel after washing away excess cells; (e) tubules; (f) branched tissues 24 h after addition of EGF to the sample; (g) one branched tissue stained for nuclei with Hoechst 33258; (h) frequency map depicting quantification of 50 branched tissues. Scale bars, 200 µm (a–f) and 50 µm (g,h).