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Overview of CocE and thermostabilizing mutations. Modeling of the L169K mutation onto the structure of CocE-T172R/G173Q shows how these stabilizing mutations cluster on H2 of Domain I (lighter space filled model), Domain II (helical region) and Domain III (darker space filled model) are shown. A portion of the DTT-carbonate adduct observed in the active site of our crystal structures is shown as ball and stick figures.
