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. 2010 Aug 11;5(8):e12018. doi: 10.1371/journal.pone.0012018

Figure 7. CHL1 deficiency results in the increased degradation of the SNARE complex proteins under elevated temperatures.

Figure 7

CHL1+/+ and CHL1−/− brain homogenates either freshly prepared or pre-incubated at 30°C for 30 minutes were probed by Western blot with the indicated antibodies. Note a more pronounced decline in the levels of syntaxin1B, SNAP25 and VAMP2, but not synaptophysin in heat-exposed CHL1−/− versus CHL1+/+ brain homogenates. Levels of Hsc70, CSP and αSGT are similar or higher in CHL1−/− versus CHL1+/+ brain homogenates exposed to 30°C. Actin served as a loading control. Graphs show mean optical density of the blots ± SEM (n = 6) with optical density in non-treated homogenates set to 100%. *, § p<0.05, paired t-test (CHL1−/− versus CHL1+/+ heat-treated brain homogenates (*), and non-treated versus treated groups of the same genotype (§) were compared).