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. 2010 Aug 11;5(8):e12018. doi: 10.1371/journal.pone.0012018

Figure 9. CHL1 deficiency results in aggregation and lysosome targeting of VAMP2 and SNAP25.

Figure 9

A - Protein aggregates and total protein from CHL1+/+ and CHL1−/− brains were probed by Western blot with the indicated antibodies. Note that higher levels of VAMP2 and SNAP25 are present in aggregates from CHL1−/− brain homogenates. Graph shows mean protein levels in aggregates ± SEM (n = 6) normalized to the total protein level. *p<0.05, paired t-test. B - CHL1+/+ and CHL1−/− lysosomes were probed by Western blot with antibodies against the lysosome associated proteins Lamp2b, SNAP25, VAMP2 and syntaxin1B. L1 served as a loading control. Graphs on the right show mean levels ± SEM of SNAP25, VAMP2 and syntaxin1B in lysosomes with levels in CHL1+/+ lysosomes set to 100%. Note similar levels of Lamp2b in CHL1+/+ and CHL1−/− lysosomes indicating similar efficiency of lysosome isolation. Graph (below) shows mean levels ± SEM of acidic phosphatase activity in CHL1+/+ and CHL1−/− lysosomes that was similar in lysosomes of both genotypes, again indicating that similar levels of lysosomes were isolated. Levels of SNAP25, VAMP2, syntaxin1B, but not L1 are increased in CHL1−/− lysosomes. *p<0.05, paired t-test.