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. Author manuscript; available in PMC: 2010 Aug 11.
Published in final edited form as: Biochemistry. 2008 Sep 25;47(42):11031–11040. doi: 10.1021/bi8010169

FIGURE 7. The effects of hydroxylamine on the kinetics ssrA-dabsyl degradation.

FIGURE 7

A) Time course of ClpPΔN degradation of ssrA-dabsyl. B) Rate constant of ssrA-dabsyl degradation by ClpPΔN as a function of hydroxylamine concentration. C) Time course of wild-type ClpP degradation of ssrA-dabsyl. D) Rate constant of ssrA-dabsyl degradation by wild-type ClpP as a function of hydroxylamine concentration. Reaction conditions: 10 μM ssrA-dabsyl with 0.125 μM ClpP tetradecamer. Traces shown are the average of two trials, and error bars represent the standard deviation of two trials. For (C) and (D) rates are shown as a fraction of the initial rate at 0 M NH2OH.