Figure 4. Fas-defective DC induce enhanced development of hapten-specific CD8⁺ T cells and CHS responses in wild type recipient mice.

(A) DC purified from DNFB-sensitized wild type (■) or lpr (●) mice were transferred by intradermal injection into naïve wild type mice. Five days later DC recipient or naïve (□) mice were challenged with 0.2% DNFB and the change in ear thickness (swelling) was measured at 24 h intervals. The mean increase in ear thickness ± SEM is shown for each group of three mice. *P < 0.01 by two-tailed Students’ t test when compared to recipients of wild type DC. The experiments were repeated two times with similar results.

(B) CD8⁺ T cells were enriched from LNC of naïve, wild type DC recipient (WT DC) or lpr DC recipient (lpr DC) mice on day +5 (□) and day +7 (■) post-transfer. CD8⁺ T cell aliquots (5 × 10⁵ cells) were cultured with DNBS-labeled splenocytes (5 × 10⁵ cells) on anti-IFN-γ mAb-coated ELISPOT plates. Numbers of IFN-γ producing CD8⁺ T cells were evaluated by ELISPOT. ND – not detected. *P < 0.05 by two-tailed Students’ t test as compared to recipients of wild type DC. The experiments were repeated two times with similar results.