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. 2010 Jul 2;11(8):640–646. doi: 10.1038/embor.2010.93

Figure 3.

Figure 3

Interferon-β inhibits interleukin-1β production in response to Mycobacterium, independent of suppressor of cytokine signalling 1. (A) Mouse DCs were pretreated with IFNγ for 3 h and stimulated with MTB overnight, LPS for 3 h, followed by MSU crystals or alum overnight. Supernatants were then analysed by ELISA for IL-1β secretion. *P<0.05. (B) Mouse DCs were pretreated with IFNγ or 100 U/ml IFNβ for 3 h, stimulated with MTB overnight and IL-1β was measured by ELISA. *P<0.05. (C) IFNγ−/− and IFNγ−/−SOCS1−/− macrophages were pretreated with IFNβ for 1–16 h, followed by LPS stimulation for 3 h. Western blot analysis was performed for pro-IL-1β showing a decrease due to IFNβ, but did not return to levels seen without IFNβ. (D) Macrophages were treated with IFNβ for the indicated times and then analysed by immunoblot using antibodies specific for pSTAT1, STAT1 and tubulin. Unlike pretreatment with IFNγ, pSTAT1 was not prolonged in SOCS1-deficient cells. DC, dendritic cell; ELISA, enzyme-linked immunosorbent assay; IFNγ, interferon-γ; IL-1β; interleukin-1β; LPS, lipopolysaccharide; MDP; muramyl dipeptide; MSU, monosodium urate; MTB, Mycobacterium tuberculosis; SOCS1, suppressor of cytokine signalling 1; STAT1, signal transducer and activator of transcription 1.