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. Author manuscript; available in PMC: 2010 Aug 12.
Published in final edited form as: Traffic. 2009 May 8;10(8):1128–1142. doi: 10.1111/j.1600-0854.2009.00941.x

Figure 3.

Figure 3

The pIgR cytoplasmic tail of pIgR contains a putative apical targeting signal.

A) Apical transcytosis of iodinated pIgA in MDCK cells expressing pIgR-WT or the C-terminal truncated mutants pIgR-725t, pIgR-737t and pIgR-747t. The experiment was repeated at least 5 times in triplicate for each mutant, and one representative experiment is shown.

B) Basolateral endocytosis of iodinated pIgA at 5 min mediated by the pIgR-WT or the C-terminal truncated mutants pIgR-725t, pIgR-737t and pIgR-747t. The experiment was performed twice in triplicate, and data from both experiments were combined. Values are mean ± SD.

C) Post-endocytic traffic (combined step 2 and 3 of pIgA apical transcytosis) of iodinated pIgA in MDCK cells expressing pIgR-WT or the C-terminal truncated mutants pIgR-725t, pIgR-737t and pIgR-747t. The experiment was repeated 3 times in triplicate for each mutant, and one representative experiment is shown.