Figure 6.

The 731-741 pIgR sequence contains an apical targeting signal.

A) Basolateral endocytosis of CD7 and CD7-pIgR^731-741. The experiment was performed twice in triplicate, and data from both experiments were combined. Values are mean ± SD.

B) Apical transcytosis of iodinated anti-CD7 monoclonal antibody endocytosed from the basolateral surface of MDCK cells expressing the wild-type CD7 or the CD7-pIgR^731-741. The experiment was performed four times in triplicate, and one representative experiment is shown.

In C) is presented the apical transcytosis of the same experiment shown in B).

D) Intracellular accumulation of pIgA at the end of the transport assay presented in A) and B).