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. Author manuscript; available in PMC: 2010 Aug 12.
Published in final edited form as: Nat Struct Mol Biol. 2009 Nov 8;16(12):1294–1301. doi: 10.1038/nsmb.1704

Figure 4.

Figure 4

36CL binding measured with equilibrium dialysis. Circles: binding to WT CLC-ec1; triangles: binding to the E148A mutant and squares: binding to the Y445A mutant. Solid lines are the best fits to 36CLbound= 36CLmax/(1+Kd/36CLfree).