Figure 4.
Reference (black) and STD NMR difference (red) spectra of chrysophaentin A in complex with FtsZ. Spectral expansion shows non-overlapped aromatic and olefinic signals of 1 displaying the strongest enhancements upon binding to FtsZ. Samples comprising 100:1 1:FtsZ were prepared in 20 mM NaPO4, 50 mM NaCl, 5 mM MgCl2, pH 6.8, and spectra recorded at 298 K. Overlaid spectra were normalized to the signal for H-3 (δ 6.85), which gave the strongest enhancement.